Sponsored by Beximco Pharmaceuticals Ltd.
Date: 21st and 22nd December, 2019
Time: 9.00AM to 5.00PM
Venue: TBA
Registration Fee: BDT 12,000
Those who are interested will need to fill up an online registration form before finalizing the payment at the Department of Pharmacy, Brac University. The form is available at
https://qrgo.page.link/jEdJq.
Please be informed that only those who complete their payment will receive confirmation of their registration for the course, and that registration/payment will be done according to a first-come first-serve basis.
Interested participants are advised to come to the Department of Pharmacy, Brac University for making the payment for this short course. For any payment related issues, contact through following:
Students (only) who register by 5th December, 2019 will be eligible for a special early bird discount, which is 50% off the standard charge.
All other interested participants who register by 5th December, 2019 will be eligible for a special early bird discount, which is 20% off the standard charge.
If you have any further queries regarding the course, please contact +8801675179821.
Genetic manipulation of biological systems has long been an essential component of a molecular biologist’s toolbox. Genome editing enables us to manipulate gene expression in a variety of animal models and target cell types. The CRISPR-Cas9 technology revolutionized the field of genome engineering and simplified the investigation of gene function. It is now helping researchers develop potential therapies against a wide range of diseases. This technology can be harnessed to introduce any desired sequence change including insertion, deletion, and point mutations at a precise location in the genome.
This course will cover the general principles of CRISPR genome editing, including design, choice of format, delivery, efficiency, and specificity of CRISPR reagents. We will discuss the steps in a typical genome editing experiment and associated quality control parameters. We will examine transfection/transduction/nucleofection strategies, mutation detection assay, clonal isolation, genotyping, and validation. We will also explore applications of genome editing in development of disease models, generation of reporter cell lines, gene therapy, and high throughput approaches for target identification and validation. Exciting, new developments in the field such as base editing and epigenome editing will also be reviewed. This course will, thus, provide an overview of a ground-breaking technology and enable attendees to undertake gene editing experiments on their own.
1. Introduction to CRISPR-Cas9 gene editing technology
2. CRISPR-mediated gene modulation
3. Targeted integration using CRISPR
4. CRISPR experimental workflow
5. Bioinformatics for genome editing
6. Case studies in cell line engineering
M. Zulfiquer Hossain, Ph.D.
Research Technology Specialist, Advanced Cell Models and Analysis
Academic Research Solutions, North America
MilliporeSigma
A business of Merck KGaA, Darmstadt, Germany &
Associate Professor (on leave)
Department of Pharmacy
Brac University
Dr. Hossain received his B.S. in Biology and Biochemistry from Brandeis University and his Ph.D. in Molecular Medicine from the University of Maryland under the Graduate Partnerships Program (GPP) with the National Institutes of Health (NIH). He has over 10 years of biomedical research experience, both as a pre-doctoral fellow at the NIH, where he discovered novel roles for β-catenin and TCF in T cell development, function, and aging, and as a post-doctoral fellow at the Johns Hopkins University, where he identified novel DNA-damaging polyphenols in foods and flavorings. He brings extensive knowledge of molecular, cancer and stem cell biology, epigenetics and immunology, as well as teaching experience as an Associate Professor at Brac University. As a Research Technology Specialist with MilliporeSigma, Dr. Hossain supports research projects that use cutting-edge functional genomics, proteomics, epigenetics, and stem cell technologies.